Adult dorsal root ganglia neurons are among the few adult neuronal cell types that can be purified and grown relatively easily in dissociated cell culture.Here top we describe a procedure for the isolation and growth of dissociated adult mouse Dishwasher Basket Plate Rack DRG neurons using Percoll gradients and a chemically defined medium.These cul
In the nervous system, the specific identity of a neuron is established and maintained by terminal selector transcription factors that directly activate large batteries of terminal differentiation genes and positively regulate their own expression via feedback loops.However, how this is achieved in a reliable manner despite noise in gene expression
Transmission expansion planning Muffin Tins is a complex problem that deals with the selection of new transmission lines that guarantee meeting future demand/generation and technical limits with the minimal investment cost.The transmission expansion planning problem has been solved through approaches and techniques aimed at reducing the computation
Retrieval of forest biophysical properties using airborne LiDAR is known to differ between leaf-on and leaf-off states of deciduous trees, but much less is understood about the within-season effects of leafing phenology.Here, we compare two LiDAR surveys separated by just six weeks in spring, in order to assess whether LiDAR variables were influenc
Benign multicystic peritoneal mesothelioma (BMPM) is a rare neoplasm that is difficult to distinguish from other cystic neoplasms radiologically.A 41-year-old female patient was admitted to the emergency service with a complaint of abdominal pain.After the top physical examination, and radiological imaging, ovarian torsion was assumed, and a gyneco